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To prevent auto-oxidation or ex vivo oxidation of the tissue, homogenization was carried out at 4C in lysis buffer containing 7M urea, 2M thiourea, 4% CHAPS, 20mM DTT, 40mM Tris HCl (pH 7.4) and 1mM PMSF

S., Takagi, S., Dohmae, N

Tian Y, Yang D

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